Molecular markers of insertion type in bacterias of the Moraxellaceae and Helicobacteraceae (phylum Proteobacteria) families

Authors

  • Ania Margarita Cutiño Jiménez Facultad de Ciencias Naturales y Exactas, Universidad de Oriente, Santiago de Cuba
  • Lianne Barrera Roca Facultad de Ciencias Naturales y Exactas, Universidad de Oriente, Santiago de Cuba
  • Vivian de la Puente López Centro Nacional de Electromagnetismo Aplicado, Universidad de Oriente, Santiago de Cuba
  • Heidy Annia Peña Cutiño Clínica Estomatológica “Fé Dora”, Santiago de Cuba

Keywords:

molecular markers, Moraxellaceae, Helicobacteraceae, Moraxella, Acinetobacter, Psychrobacter, Helicobacter, DNA polimerase I, DNA polimerase III.

Abstract

A study with the use of Gupta methodology was carried out in the Biology and Geography Department of Oriente University in Santiago de Cuba, to determine molecular markers of insertion type in sequences of the DNA polimerase I proteins and DNA polimerase III (alpha subunity), obtained from international databases and later on aligned with the ClustalX2 program.  The Moraxellaceae and Helicobacteraceae families have been broadly studied, because they comprise pathogen agents that cause numerous diseases in humans, but few investigations have been directed to the identification of the molecular characteristics that can distinguish their members from other groups of bacterias; so these results constitute a contribution to the knowledge of genetics and biochemistry of these families and provide molecular tools for the taxonomic classification and the diagnosis of pathogen species.

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Author Biography

Ania Margarita Cutiño Jiménez, Facultad de Ciencias Naturales y Exactas, Universidad de Oriente, Santiago de Cuba

Profesor auxiliar y Coordinadora de la carrera Biología. Departamento de Biología, Facultad de Ciencias Naturales y Exactas, Universidad de Oriente.

References

1. Teixeira LM, Merquior VLC. The Family Moraxellaceae. En: Rosenberg E, DeLong EF, Lory S, Stackebrandt E, Thompson F. The Prokaryotes. Berlin: Springer Heidelberg; 2014. p. 443-76.

2. Perez AC, Pang B, King LB, Tan L, Murrah KA, Reimche JL, et al. Residence of Streptococcus pneumoniae and Moraxella catarrhalis within polymicrobial biofilm promotes antibiotic resistance and bacterial persistence in vivo. Pathogens and Disease. 2014; 70(3): 280-8.

3. Deschaght P, Janssens M, Vaneechoutte M, Wauters G. Psychrobacter isolates of human origin, other than Psychrobacter phenylpyruvicus, are predominantly Psychrobacter faecalis and Psychrobacter pulmonis, with emended description of P. faecalis. Int J Syst Evol Microbiol. 2012; 62(Pt 3): 671-4.

4. Prado A, Arias NL, Chávez M, Cabrera CE, Gómez RF. Caracterización fenotípica de aislamientos de Acinetobacter baumannii en una institución de salud de alta complejidad de Cali. Biomédica. 2014; 34: 101-7.

5. Mitchell HM, Rocha GA, Kaakoush NO, O’Rourke JL, Queiroz DM. The family Helicobacteraceae. En: Rosenberg E, DeLong EF, Lory S, Stackebrandt E, Thompson F. The Prokaryotes. Berlin: Springer Heidelberg; 2014. p. 337-92.

6. Ledesma Z, Gutiérrez B, Cirión G, Lemus MV, Sanabría J, Romero T, et al. Diagnóstico histológico de la infección por Helicobacter pylori en Pinar del Río, Cuba. Vaccimotot. 2010 [citado 25 May 2017]; 19(2):4. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2010000200001

7. Gupta RS. Identification of Conserved Indels that are Useful for Classification and Evolutionary Studies. En: Michael Goodfellow IS, Jongsik C. Methods in Microbiology. Vol. 41. New York: Academic Press; 2014. p. 153-82.

8. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, et al. Clustal W and Clustal X version 2.0. Bioinformatics. 2007; 23(21): 2947-8.

9. Hall B. Comparison of the accuracies of several phylogenetic methods using protein and DNA sequences. Mol Biol Evol. 2005; 22(3): 792-802.

10. Yi H, Yong D, Lee K, Cho Y-J, Chun J. Profiling bacterial community in upper respiratory tracts. BMC Infectious Diseases. 2014; 14(1): 583.

11. Medell M, Hart M, Duquesne A, Espinosa F, Valdés R. Nosocomial ventilator-associated pneumonia in Cuban intensive care units: bacterial species and antibiotic resistance. MEDICC Rev. 2013; 15(2): 26-9.

12. Méndez Leyva, Begué Dalmau N, Tamayo Heal S, Alonso Vázquez A, Fías Chan NV. Infección por Helicobacter pylori en el municipio de Palma Soriano durante el período 2008-2014. MEDISAN. 2016 [citado 25 May 2017]; 20(11): 2339-44. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1029-30192016001100002

13. Salama NR, Hartung ML, Müller A. Life in the human stomach: persistence strategies of the bacterial pathogen Helicobacter pylori. Nature reviews Microbiology. 2013; 11(6): 385.

14. Bento-Miranda M, Figueiredo C. Helicobacter heilmannii sensu lato: An overview of the infection in humans. World J Gastroenterol. 2014; 20(47):17779-87.

15. Gupta RS, Sethi M. Phylogeny and molecular signatures for the phylum Fusobacteria and its distinct subclades. Anaerobe. 2014; 28: 182-98.

16. Ho J, Adeolu M, Khadka B, Gupta RS. Identification of distinctive molecular traits that are characteristic of the phylum “Deinococcus-Thermus” and distinguish its main constituent groups. Systematic and Applied Microbiology. 2016; 39(7):453-63.

Published

2018-01-11

How to Cite

1.
Cutiño Jiménez AM, Barrera Roca L, de la Puente López V, Peña Cutiño HA. Molecular markers of insertion type in bacterias of the Moraxellaceae and Helicobacteraceae (phylum Proteobacteria) families. MEDISAN [Internet]. 2018 Jan. 11 [cited 2025 Jun. 4];22(1). Available from: https://medisan.sld.cu/index.php/san/article/view/1396

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Section

Original Articles