Morphological classification of endothelial cells of veins from the human umbilical cord in digital images of in vitro cultures

Authors

  • Miriela Milagros Escobedo Nicot Universidad de Oriente, Santiago de Cuba
  • Silena Herold García Universidad de Oriente, Santiago de Cuba
  • Ligia Ferreira Gomes Universidade de São Paulo
  • Wilkie Ernesto Delgado Font Universidad de Oriente, Santiago de Cuba
  • Camila Machado Universidade de São Paulo
  • Elisângela Monteiro Pereira Universidade Federal de Alfenas, Minas Gerais

Keywords:

morphological analysis, endothelial cells, in vitro culture, angiogenesis.

Abstract

A descriptive and cross-sectional study was carried out from July to October, 2017, by specialists of the Oriente University  and  the University of Sao Paulo, Brazil, to analyze from the morphological point of view endothelial cells of the human umbilical cord veins, which were present in digital images of 2D vitro culture, treated with the β2GPI. The cellular supervised classification was proposed considering 3 classes: circular, distorted elongated and distorted  not very elongated, according to the coefficients of elliptic and circular shapes, all that allowed to identify outstanding cellular forms. To compare the results of the control and treated samples, the intervals of confidence were calculated for each of the classes, with a 95% level of confidence. It was concluded that the analysis of the morphological disorders in vitro can be used in early 2D cultures (24 and 48 hours) for the quantification of the angiogenesis. 

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Published

2018-02-13

How to Cite

1.
Escobedo Nicot MM, Herold García S, Ferreira Gomes L, Delgado Font WE, Machado C, Monteiro Pereira E. Morphological classification of endothelial cells of veins from the human umbilical cord in digital images of in vitro cultures. MEDISAN [Internet]. 2018 Feb. 13 [cited 2025 Sep. 3];22(2). Available from: https://medisan.sld.cu/index.php/san/article/view/1745

Issue

Vol. 22 No. 2 (2018): MEDISAN

Section

Original Articles

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