Histomorphometric study of the cerebellar cortex with digital holographic microscopy
Keywords:
cerebellar cortex, histomorphometric study, digital holographic microscopy.Abstract
A descriptive and cross-sectional study of the human cerebellar cortex, from the histomorphometric point of view, was carried out from January to September, 2015, using the digital holographic microscopy installed in the Digital Holography Department of Oriente University inSantiago de Cuba, aimed at making measurings that allowed to establish comparisons with other studies. The calculations showed the thickness of the molecular and granular layers, the area, perimeter, greatest and smallest diameters of the body and Purkinje cells nucleus. Also, holographic images in three dimensions were taken, that facilitated to conclude the existence of certain parameters by means of this procedure, those that had not been notified and are of interest in the histological study of the cerebellar cortex.
Downloads
References
2. Bustamante J. Neuroanatomía funcional y clínica. 4 ed. Bogotá: Celsus; 2007.
3. Xiao J, Cerminara NL, Kotsurovskyy Y, Aoki H, Burroughs A, Wise A, et al. Systematic regional variations in Purkinje cell spiking patterns. PLos One. 2014 [citado 14 Ago 2016]; 9(8). Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140808/
4. Kirschen MP, Chen SH, Desmond JE. Modality specific cerebro-cerebellar activations in verbal working memory: an fMRI study. Behav Neurol. 2010 [citado 14 Ago 2016]; 23(1-2): 51–63. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2944406/pdf/BN-2010-23-1-587450.pdf
5. Marvel CL, Desmond JE. The contributions of cerebro-cerebellar circuitry to executive verbal working memory. Cortex. 2010 [citado 14 Ago 2016]; 46(7): 880–95. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2872048/
6. Stoodley CJ. The cerebellum and cognition: evidence from functional imaging studies. Cerebellum. 2012; 11(2): 325-65.
7. Mir TA, Shinohara H. Label-free observation of three-dimensional morphology change of a single PC12 cell by digital holographic microscopy. Anal Biochem. 2012; 429(1): 53-7.
8. Samar ME, Ávila RE. Histología humana clínicamente orientada: tejidos y sistemas. 3 ed. Córdoba: Samar; 2010.
9. Storniolo Squintone A, Guerini JC, Fonseca IB, Samar ME, Spitale LS. Estudio morfométrico comparativo de la corteza cerebelosa humana en dos grupos etarios. Int J Morphol. 2012 [citado 3 Mar 2016]; 30(3). Disponible en: http://www.scielo.cl/scielo.php?pid=S0717-95022012000300009&script=sci_arttext
10. Mirarab Razi E, Ghafari S, Hojati V, Jafar Golalipour M. Effect of gestational diabetes on neuronal cells in rat cerebellum in early postnatal life. Int J Morphol. 2014 [citado 3 Mar 2016]; 32(2): 420-5. Disponible en: http://www.intjmorphol.com/wp-content/uploads/2015/06/art_06_322.pdf
11. Golalipour MJ, Kafshgiri SK, Ghafari S. Gestational diabetes induced neuronal loss in CA1 and CA3 subfields of rat hippocampus in early postnatal life. Folia Morphol (Warsz). 2012; 71(2):71-7.
12. Engbers JD, Fernandez FR, Turner RW. Bistability in Purkinje neurons: ups and downs in cerebellar research. Neural Netw. 2013; 47: 18-31.
13. Nuket Gocmen-Mas H, Karabekir S, Yilmaz Kusbeci O, Sahin B, Ertekin T, Bas O, et al. Morphometric analysis of hemicerebellar asymmetryn with central vertigo cases: a stereological study. Int J Morphol. 2010 [citado 3 Mar 2016]; 28(2). Disponible en: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200048&lng=es&nrm=iso&tlng=en
14. Stoodley CJ, Valera EM, Schmahmann JD. An fMRI study of intra-individual functional topography in the human cerebellum. Behav Neurol. 2010 [citado 3 Mar 2016]; 23(1-2): 65-79. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776583/pdf/BN-2010-23-1-840942.pdf
15. Fawcett W. Tratado de Histología. 12 ed. Madrid: McGraw-Hill Interamericana; 1995.
16. Mescher AL. Junqueira's Basic Histology: text & atlas. 12 ed. New York: McGraw-Hill; 2009.
Published
How to Cite
Issue
Section
License
All the articles can be downloaded or read for free. The journal does not charge any amount of money to the authors for the reception, edition or the publication of the articles, making the whole process completely free. Medisan has no embargo period and it is published under the license of Creative Commons, International Non Commercial Recognition 4.0, which authorizes the copy, reproduction and the total or partial distribution of the articles in any format or platform, with the conditions of citing the source of information and not to be used for profitable purposes.